In vitro Callus Induction Potentials of Wheat Genotypes using Mature Embryo as ex-plant source under different levels of Polyethylene glycol (PEG

M.K. Haruna¹, C.U. Aguoru², C.C. Iheukwumere², C.I.C. Ogbonna³, and I.D. Salisu⁴

¹Department of Biology, Federal University Gashua, Yobe State, Nigeria.

²Department of Botany, University of Agriculture, Makurdi, Nigeria.

³Department of Biotechnology, University of Jos, Nigeria.

⁴Department of Biotechnology, Jigawa Research Institute, Kazaure.

Email: mokharry2013@gmail.com           

ABSTRACT

This work was carried out at Jigawa Research Institute Biotechnology Laboratory Kazaure to determine callus induction potentials of 16 wheat genotypes using matured embryo as ex-plant source under different (PEG) levels. The experiment had six treatments (0%, 5%, 10%, 15%, 20% and 25%) including control with each replicated three times and the necrotic percentage taken showed varieties 8(53.3%), 7(60%) and 28(61.1%) had minimal necrotic callus, while ANOVA shows that there is significance difference between the treatments percentage values (P<0.05), while there was positive correlation among the genotypes. Also the total number of callus recorded for each variety across treatment showed an encouraging callus production in varieties 8(38), 7(34), 2(32) and 6(31), however (ANOVA) shows significant difference between the treatment  mean values (p<0.05) while Pearson correlation coefficient among the genotypes indicated that there was positive correlation.

Keywords: Callogenesis, drought stress, embryo culture, tissue culture, Triticum aestivum L.