PHYTOCHEMICAL SCREENING AND SCAVENGING EFFECTIVENESS OF THE LEAF OF TERMINALIACATAPPALINN
1Umar Dan Azumi, 1Tijjani M.AMohammed G.T.2Braimah H.Y.
1Department of Chemistry, University of Maiduguri, Maiduguri, Borno State, Nigeria.
2Department of Pharmacuetical Chemistry, University of Maiduguri.
Email:
mustaphatijjani22@yahoo.com
Abstract
Fresh leaf of Terminaliacatappa was collected from Bolori ward Maiduguri Borno state and it was identified by Professor S. S. Sunusi Department of Biological Science Faculty of Science, University of Maiduguri. One thousand grammes (1000g) of the powdered leaf of Terminaliacatappa was extracted with methanol using cold infusion (maceration) method. Eighty three point eight two grammes (83.82g) of the dark green in colour gummy in texture of methanol crude extract was obtained, which was further partitioned with n-hexane, ethyl acetate, n-butanol and water to give n-hexane portion (1.638% W/W), dark green in colour, oily in texture, ethyl acetate portion (0.075% W/W), black in colour, gummy in texture, n-butanol portion (0.777% W/W), brown in colour, oily in texture and finally aqueous portion (2.997% W/W), dark brown in colour, powdered in texture. Preliminary phytochemical screening of the methanol crude extract and partitioned portions revealed the presence of some secondary metabolites such as cardiac glycoside, flavonoids, saponins, terpenoids, tannins and alkaloid. The antioxidant activity was carried out on the methanol extract and partitioned portions. The methanol extract showed the percentage inhibitions of 98.25 at 10ug/ml 97.40 at 20µg/ml 96.94 at 30µg/ml 96.63 at 40µg/ml and 97.10 at 50µg/ml, n-butanol portion showed the percentage inhibitions of 95.75 at 10ug/ml 96.40 at 20µg/ml 96.15 at 30µg/ml 96.40 at 40µg/ml and 96.15 at 50µg/ml, n-hexane portion showed the percentage inhibitions of 95.50 at 10ug/ml 95.65 at 20µg/ml 95.80 at 30µg/ml 95.75 at 40µg/ml and 95.75 at 50µg/ml, ethyl acetate portion showed the percentage inhibitions of 78.35 at 10ug/ml 87.65 at 20µg/ml 95.00 at 30µg/ml 94.75 at 40µg/ml and 94.70 at 50µg/ml. Finally aqueous portion showed the percentage inhibitions of 94.40 at 10ug/ml 95.10 at 20µg/ml 96.00 at 30µg/ml 95.50 at 40µg/ml and 96.05 at 50µg/ml. The methanol extract showed promising antioxidant activities at various concentrations when compared with the partitioned portions.