INFLUENCE OF BRADYRHIZOBIUM JAPONICUM, MYCORRHIZA AND POULTRY MANURE ON NODULATION, NITROGEN UPTAKE, GROWTH AND YIELD OF SOYBEAN (GLYCINE MAX L.) UNDER MANURE APPLICATION
1*Adigun M.O., 2Babalola O.A & 3Abiola I.O.
1*Department of Biological Sciences, Crawford University, Faith City, Igbesa, Ogun State,
2Department of Soil Science & Land Management, Federal University of Agriculture, Abeokuta, Nigeria
3Crop Production Technology Department, Federal College of Forestry, Jericho, Ibadan
Abstract
This study was carried out to assess the effects of Bradyrhizobium japonicum, mycorrhiza fungi and poultry manure on nodulation, growth and yield of soybean under manure application. Four types of treatments were used (poultry manure, Bradyrhizobium inoculation, mycorrhiza and Single Super Phosphate) and were added to each experimental sub-unit. Different rates of poultry manure were added 0 t ha-1, 5 t ha-1 and 10 t ha-1 and left for two weeks to decay while Rhizobium inoculation comprises of inoculated and un-inoculated, the plot was sub-divided into 36 units and arranged in randomized completed block design. Leaf area, number of leaves, plant height and canopy diameter were determined at 4, 6, 8, 10, 12 and 14 Week After Planting. Number and weight nodules, fresh and dry weight of shoot and root were determined at the 6th and 8th weeks after planting and grain yield were determined at harvest. Soil samples were also collected before and after planting for laboratory analysis. The poultry manure used was also analyzed for the amount of N: P: K present. Rhizobium inoculation with addition of mycorrhiza fungi significantly increased the nitrogen fixation of the soybean and also helps the plant to withstand or tolerate water stress. Combination of Brad+Myc+5PM had effect on leaf area of plant. Myc+5PM had significant effect on stem girth of soybean and combination of Brad+Myc+5PM significantly increased the height of plant. The application of SSP helps to provide phosphorus to the plant. The 5 kg\ha and 10 kg\ha of poultry manure and mycorrhiza fungi significantly affects and improves the growth, nitrogen uptake and yield of soybean. Bradyrhizobium also helps in effective nodulation of the soybean.
Keywords: Nodulation, Rhizobium, Nitrogen fixation, Water stress, Soybean, Yield.