ANTIBACTERIAL AND IDENTIFICATION OF COMPOUNDS IN THE LEAVES EXTRACTS OF BRIDELIA FERRUGINEA

¹Abubakar, L., ²John, M., ¹Muhammad, M.U., ¹Muhammad, S and ¹Adamu, S.M.

¹Department of Chemistry, Shehu Shagari College of Education, Sokoto.

²Sokoto State Teachers Service Board, Sokoto

E-mail: Lawaligada@gmail.com

ABSTRACT

Bridelia ferruginea is extensively used in Nigeria traditional and folk one medicines to cure various human ailments. The preliminary phytochemical screening of the plant parts revealed the presence of tannins, saponins, flavonoids and alkaloids. The in vitro antibacterial studies were carried out on both crude extracts and fractions obtained after column chromatography and medically important bacterial strain including Escherichia coli, Staphylococcus aureus, Micrococcus species and Pseudomonas aeruginosa for different concentration of extracts 30mg/ml, 60mg/ml, 90mg/ml, and 120mg/ml. The results of antibacterial assay revealed that both crude extracts and fractions showed good inhibitory activity against all the tested bacteria compared with standard antibiotics. The active fractions gave four spots on the Thin Layer Chromatogram. The UV spectroscopy indicated the presence of open chain diene, ά and β unsaturated aldehyde and also aromatic ring. The IR indicated the presence of different functional groups such as C-N, O-H, N-H and C-H. The GC-MS analysis indicated the presence of Psilocybine and Tolazimide. The detection of other phytochemicals metabolites coupled with antibacterial activities of the plant calls for further work on the plant and its active compound towards development of new drugs for the benefit of humanity.