Bacteriological Examination and Antibiotic Resistance Profile of Bacteria Isolated from Bottled Water Sold in Amai, Delta State.

^*1Tanimowo, W.O., ¹Kolagbodi R., ²Ukolobi O. and ²Osaide S.

¹Department of Biological Sciences, Novena University, Ogume, Delta State, Nigeria.

²Department of Microbiology, Delta State University, Abraka, Delta State, Nigeria.

E-mail: jesumowo0630@yahoo.com

ABSTRACT

This study was carried out to investigate the microbiological quality as well as the antibiotic resistance profile of bacterial species in some bottled water sold in Ukwuani Local Government Area of Delta State. The presence of heterotrophic plate count from six (6) commercially available bottled water were examined using standard pour plate and spread plate methods, while the antibiotic resistance was investigated using disc diffusion method. The results obtained indicated that 16.7% and 83.3% of the samples showed heterotrophic plate (HPC) count within the range of less than 1(CFU/ml) and 1- 250 (CFU/ml), respectively, and 0% sample showed greater than 250 (CFU/ml) in heterotrophic plate counts. The results also showed that 83.3% klebsiella, 83.3% Staphylococcus aureus, 50% Salmonella, 50% Serratia, 66.7% Escherichia coli and 16.7% Streptococcus, were isolated from the water samples. All the bacterial isolates were resistant to the antibiotics tested. Since some bacterial species have developed resistance and are still developing resistance against certain antibiotics which were once known to be active against such bacterial species,  it is therefore recommended that good treatment techniques that would eliminate all forms of microorganisms that could cause water related diseases be used to treat bottled water before distributing to the consumer populations.