INVIVO EFFECT OF CO-TRIMOXAZOLE ON PLASMA AMINOTRANSFERASE (AST) (EC 2.6.1.1) AND ALANINE AMINOTRANSFERASE (ALT) (EC. 2.6.1.2)

ACTIVITY OF WISTAR ALBINO RAT

Nwachoko, N. and Laya, R.O.

Chemistry Department (Biochemistry Option)

Rivers State University of Science and Technology, Port Harcourt, Nigeria.

E-mail: blessedconfidence@yahoo.com

ABSTRACT

The assay of plasma alanine aminotransaminase (ALT) and aspartate aminotransaminase (AST) are known to be useful in assessing the functional integrity of the liver. Thus, this investigation considered possible invivo effect of septrin on plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) of wistar albino rat. The rates were divided into five groups (control group and experimental groups). The experimental groups were administered with serially diluted co-trimoxazole at different concentration (0.1, 0.3, 0.5, and 1.0)mg/ml. Blood samples were collected on day four and day eight for analysis. Values of AST and ALT were measured using LAB-TECH colorimeter. From the result, the mean values of AST after day 4 of administration were (36.00, 49.50, 59.00, 63.00, 63.00) U/l and after day 8 (44.00, 49.50, 53.00, 76.00, 76.00) U/l for group A, B, C, D and E respectively. Also the mean values of ALT after day 4 of administration were (12.00, 6.00, 8.00, 6.00, 12.50) U/l and after day 8 (12.00, 10.00, 4.00, 14.50, 10.00) U/l for group A, B, C, D, and E respectively. The result showed that values of AST of the experimental rats had significant increment (P > 0.05) Compare to that of control group. ALT activity in the blood sample of the experimental rats had no significant different when compared to that of control group.