RESISTANCE PATTERN OF UROPATHOGENIC E. COLI STRAINS ISOLATED FROM A NIGERIAN HOSPITAL
1Funmilola A. Ayeni and 2Gladys O. Henshaw
Department of Pharmaceutical Microbiology, University of Ibadan, Oyo State, Nigeria
2Faculty of Pharmacy, Igbinedion University Okada, Edo State, Nigeria
E mail-funmiyeni@yahoo.co.uk
ABSTRACT
Bacterial resistance to antibiotics is a global problem which results in difficulty in treatment. Surveillance study should always be carried out in different geographical location to determine current effective antibiotics against bacterial infections. Escherichia coli strains were isolated from High Vaginal Swab (HVS), Endocervical Swab (ECS), and urine samples of patients visiting Igbinedion University Teaching Hospital between April and May, 2010. Identification of E. coli strains were based on growth on selective agar media and Gram’s reaction. Susceptibility testing of these E. coli strains was done against an array of antimicrobials using the disk agar diffusion method and Minimum Inhibitory Concentration (MIC) determination by macrodilution method. Sixteen E. coli strains were isolated and identified. The percentage of resistant to Nitrofurantoin, Ciprofloxacin, Ampicillin, Amoxiclav, Chloramphenicol, Nalidixic acid, Sulphamethazole, Gentamycin were 18.75%, 18.75%, 37.5%, 18.75%, 62.5%, 12.5%, 37.5%, and 31.25% respectively. The strains were highly resistant to Chloramphenicol while susceptible to Nalidix acid. The result of disc diffusion method also agrees with MIC method. The resistance observed in this study to some antibiotics could be because of inappropriate and incorrect administration of these antimicrobial. To avoid this, there should be strict control in the use of these antibiotics.
Keywords:-Resistance, antibiotics, uropathogens, urinary tract infections