VIRUS FREE PLANTLETS PRODUCTION OF SWEET POTATO (Ipomea batata (L.) Lam) THROUGH TISSUE CULTIVATION AND MERISTEM CULTURE
1Benisheikh, A.A.G, 2Zainab .M. Aliyu, 3Zainab Tamus, 4Abdullahi Audu and 5Mala Modu
1North East Zonal Biotechnology Centre, University of Maiduguri, Borno State.
2Deparment of Biological Science
Sir Kashim Ibrahim College of Education Maiduguri, Borno State, Nigeria
3Department of Basic Studies
Mohmet Lawal College of Agriculture Maiduguri, Borno State, Nigeria
4Department of Agricultural Technology
Ramat Polytechnic, Maiduguri, Borno State, Nigeria.
5Department of Forestry and Wildlife, University of Maiduguri, Borno State, Nigeria.
ABSTRACT
Virus free sweet potato plantlet production were investigated through meristem culture of sweet potato plantlets meristem was isolated from field grown runner tip explants and cultured on modified Murashige and Skoog (1962) medium containing auxin (2,4- D) and cytokinins (KN, BA, BAP) was used to cultivate meristem culture and tissue culture of sweet potato (Ipomea batata (L.) Lam) in vitro. Concentration of sucrose was at 3% w/v level. The pH media was adjusted to 5.7 before the addition of agar 8% w/v, meristems with 2-3 leaves primordial were dissected out aseptically and inoculated to the media (various levels of hormones and in different combinations), then incubated at 27 + 20C under light intensity of 2000-3000 lux in the culture room. Treatments include 2,4-D and BA at levels of 1mg/L and 0.25 mg/L, respectively with GA3, (0.1 mg/L) produced better plantlet, but higher levels of 2,4-D with KN (2.5 mg/L) induced the meristems to form callus. After having DAS- ELISA test the in vitro grown plantlets were being used for massive micro propagation. Visual evaluation of the morphological trails of the meristem culture derived plants showed normal and free from various diseases. Gross yield was obtained in meristem derived plants over their source plants.
Recent Comments