EFFECT OF AQUEOUS EXTRACT OF DIOSCOREABULBIFERA ON SOME BIOCHEMICAL PARAMETERS IN ALLOXAN- INDUCED DIABETIC RATS.
1Luka, C.D and 2Mohammed, A
1Department of Biochemistry, University of Jos
2Department of Biochemistry, Federal University Wukari
Email:carrll42@yahoo.com,
ABSTRACT
The study was carried out to investigate the effect of the aqueous extract of Dioscoreabulbiferatuber on blood glucose, protein, albumin, bilirubin, total cholesterol;triglyceride, high density lipoprotein and enzymes activities on normal and alloxan induced diabetic rats. The aqueous extract was administered orally at a dose of 400mg/kg body weight to both normal and alloxan induced diabetic rats. Twenty adult male rats were divided into four groups of five rats each, two groups were made diabetic and the other two groups were non diabetic. One of the diabetic groups was treated with the extract and the second serves as diabetic control. The alloxan was administeredintraperitoneal at a dose of 150mg/kg per body weight. The administration of the extract lasted for 14 days. Effect of the extract on blood glucose, protein, albumin, bilirubin, total cholesterol; triglyceride and high density lipoprotein concentrations were analysed.The toxic effect of the extract was determined using biochemical enzyme markers. The photochemical screening of the aqueous, ethanol and chloroform extracts showed the presences of alkaloid, flavonoid, tannins, saponins, cardiac glycosides, resins, terpenes and steroids. Treatment with the extract showed significant (P<0.05) reduction on the blood glucose level and other biochemical parameters. Theextract possesses no toxic effect as indicated by the lowering of ALP and ALT levels and may be used for the management of diabetes mellitus.
Keywords: Dioscoreabulbifera,Alloxan, Photochemical, Diabetes Mellitus, Hypoglycaemic.