VIRUS FREE PLANTLETS PRODUCTION OF SUGAR (SACCHARUM OFFICINARUM (L)) THROUGH INVITRO MICROPROPAGATION OF SHOOT TIP CULTURE

¹Benisheikh, A. A. G, ² Mala Modu ³ Ahmadu Umoru ⁴ Fatima, B. K. Kolo ⁵Zainab Mudi Aliyu

¹North East Zonal Biotechnology Centre of Excellent P.M.B 1069 University of Maiduguri, Borno State

²Department of Forestry and Wildlife Management, University of Maiduguri

³Department of Science laboratory Technology, Ramat Polytechnic Maiduguri

⁴Department of S.L.T Ramat Polytechnic Maiduguri

⁵Dept. of Biology, Sir Kashim Ibrahim College of Education

E-mail: abbabenisheikh@yahoo.com

ABSTRACT

Standardization of protocol for virus-free sugarcane production were investigated through shoot tip culture of sugarcane was isolated from grown sugarcane tip of explants and cultured on modified Murashige and Skoog (M&S 1962) medium containing auxin (2, 4-D) and cytokinins (KN, BA, BAP) was used to cultivate shoot tip of sugarcane (L) in vitro. Concentration of sucrose was at 3% W/V level, the PH media was adjusted to 5.6 prior to the addition of agar 8% W/V. young shoot tip were cut into thin smaller pieces of 1.0 to 1.5cm length-and inoculated to the media (various levels of hormones and in different combinations), then incubated at 27 ± 2⁰C under light intensity of 2000 – 3000 Lux in the culture room. Treatments 2, 4 – D and BA at levels of 0.8mg/L and 0.25mg/L respectively with GA₃ (0.1mg/L) produce optimum multiplication. After having DAS-ELISA test the invitro grown plantlets were being used for massive micro propagation. Visual evaluation of the morphological trials of the shoot tip culture. Derived plants showed normal and free from various diseases. Gross yield was obtained in shoot tip derived plants over their source plants.

Key Words: shoot tip, virus free, plantlets production, sugarcane, invitro micropropagation, culture.