RESISTANCE PATTERN OF UROPATHOGENIC STAPHYLOCOCCAL STRAINS ISOLATED FROM OUTPATIENTS IN A NIGERIAN HOSPITAL
Funmilola A. Ayeni1*, Osagie Omoregie 1 and Misan Olley2
1Department of Pharmaceutical Microbiology, College of Pharmacy, Igbinedion University Okada
2Medical Microbiology Laboratory, Igbinedion University Teaching Hospital Okada
ABSTRACT
Bacterial resistance to antibiotics is a global problem which results in difficulty in treatment. This study determined antimicrobial resistance of staphylococci isolated from urogenital tracts of humans with a presumptive diagnosis of urinary tract infection (UTI). Forty five urogenital samples (endocervical swab, high vaginal swab and urine) were obtained from outpatients at Igbinedion University Teaching Hospital between April and May, 2010. They were processed for isolation of Staphylococcus sp. Colonies in pure culture were identified by biochemical reactions and tested for susceptibility to 9 antimicrobials using disk agar diffusion method. Minimum Inhibitory Concentration (MIC) was determined by macrodilution method. Ten isolates of staphylococci were obtained (22% of the total samples). Staphylococcus aureus was the most frequent species (70%). All the isolates were multidrug resistant with each isolate exhibiting resistance to at least 5 antimicrobials. All the isolates had 100% resistance to nitrofurantoin, ampicillin, ciprofloxacin, augmentin, and ceftriazone. All coagulase negative Staphylococcus sp. strains were susceptible to doxycycline while S. aureus strains were relatively susceptible to TMP/SMX. The study reports the alarming antimicrobial resistance of members of the Staphylococcus genus isolated from human urogenital tract. There should be strict control in the use of antibiotics for chemotherapy of staphylococcal infections to reduce the organism’s resistance to commonly used antibiotics.
Keywords:-Resistance, antibiotics, uropathogens, multidrug resistance.