Purification and Analysis of bioactive compounds from Citrus aurantifiolia Linn stem bark by Gas- Chromatography-Mass spectrometry

Mustapha A. Tijjani1* Fanna I. Abdulrahman1,Sherrif Umar1, Fatima M. Dungus2

1Department of Chemistry, University of Maiduguri, Borno State, Nigeria

2Departement of Clinical Pharmacy, University of Maiduguri, Borno State, Nigeria

Email: mustaphatijjani22@yahoo.com

ABSTRACT

The objectives of this research are to extract the air-dried stem bark (500g) of citrus aurantifolia with methanol using cold infusion (maceration) technique, partition the extract  with solvents of graded polarities (n-hexane, ethyl acetate, n-butanol suspended in water) and phytochemically screened and fractionate and purify the n-butanol partitioned portion using a combination of column and thin layer chromatographyand finally subject the possible pure fraction(s)  to gas chromatography and mass spectrometry (GC-MS) analysis. The methanol crude extract yield 14.90% w/w dark green in colour, gummy in texture. the n-hexane partitioned portion yield 0.44% w/w  light green in colour oily paste texture, ethylacetate partitioned portion yield 5.32% w/dark brown in colour, gummy in texture, while n-butanol yield 21.04%w/w, brown in colour, gummy in texture and finally aqueous partitioned portion yield 52.08% w/w  brown in colour power in texture respectively. The presence of metabolites such as carbohydrates, cardiac-glycosides, terpeniods, flavonoids, tannins and phlobatannins were recorded in the methanol crude extract while, anthraquinones, alkaloids and saponins were not detected in methanol crude stem bark extract. Whereas cardiac-glycosides, terpeniods and flavonoids were present in n-hexane portion and n-butanol portion but carbohydrates, anthraquinones, tannins, saponins, phlobatannins and alkaloids were not found in both of the portions.. The purification of compounds was done by using a combination of column and thin layer chromatography techniques. The n-butanol partitioned portion was subjected column chromatography after, rerunning, recombination and pooling four compounds, coded Ca1, Ca2, Ca3 and Ca4 were obtained. The melting points were sharp and uncorrected. In gas chromatography and mass spectrometry (GC-MS) analysis of compound of sample Ca1 fourteen compounds were identified by comparison with the library of NIST. Among the compounds, were found to be 3, 5, 9-Trioza-5-Phosphaheptacos-18-en-1-aminium, Pentacosanoic acid, Oleic acid, 7, 8-Epoxylanostan-11-ol. Analysis of Compound of sample Ca2  shows that only one compound was identified by comparison with the library of NIST. The compound was found to be 3H-Cycloocta[c]pyran-3-one. Analysis of Compound of sample Ca3 shows eight compounds were identified by comparison with the library of NIST. Among the compounds were found to be 1, 3-Dioxane, Pregn-5-en-20-one, 9-Octadecenoic acid, Heptadecanoic acid, Epoxylanostan-11-ol. Analysis of Compound of sample Ca4  shows that eight compounds were identified by comparison with the library of NIST. Among the compounds were found to be Glycidol stearate, Andrast-4-ene-3-one, Octadecanoic acid, Dihydromorphine.. The dihydromorphine which believed to be a reduced formed of morphine with a molecular formula of C17H21NO3. It has been reported thatthe 7, 8-double bond of morphine also is not required for analgesic activity as indicated by the relative analgesic potency of dihydromorphine. Also, oxidation of the 6-OH of dihydromorphine to yield hydromorphone further increases activity.

Keywords: Citrus aurantifolia, purity, column chromatography, Mass spectrometry


ARSENIC REMEDIATION OF DRINKING WATER

Kamaru M. B1. Umar S2. Usman A. A3, Fatima L. B4

1,2,3,4Department of Science Laboratory Technology

Umaru Ali shinkafi Polytechnic Sokoto

Email: bashirkamaru@yahoo.com

ABSTRACT

In this study, the potential of limestone as an adsorbent of different particle sizes for the removal of arsenic from drinking water was investigated. Effects of various operating parameters such as particle size, contact time and adsorbent dosage ere studied using batch adsorption studies. X-ray fluorescence (XRF) was used to certify the mineral composition of limestone. This method shows maximum removal of arsenic 98.4 % under the following operating conditions: contact time 10 min, adsorbent dosage 2 g, particle size 2 µm and temperature 28 oC. Pseudo-first and pseudo-second-order kinetic models were employed to elucidate the adsorption behaviour of the system. The result of the adsorption process showed a poor correlation value with the pseudo-second-order model compared to the pseudo-first-order. This designates that the rate-determining steps in the adsorption of arsenic could be physical adsorption processes.

Keywords: Limestone, arsenic, adsorption, correlation, kinetics


STUDIES ON THE POTENTIALS OF MANGO (Magniferaindica) SEED OIL AS RAW MATERIAL FOR THE PRODUCTION OF SOAP

1*Arzika, A.T., 1Ahmad, M.B., 1Adamu, S.M., 1Bagna, E.A., 2Bashar, S.Y., 1Mohammed, S. and 3Sani, G.

1*Department of Chemistry Shehu Shagari College of Education, Sokoto, Nigeria

2Department of Chemistry, State College of Basic and Remedial Studies, Sokoto, Nigeria

3Department of Science Laboratory Technology, College of Science and Technology, Umaru Ali Shinkafi Polytechnic Sokoto, Nigeria

E-mail: arzikatambuwal1982@gmail.com

ABSTRACT

The seed oil of mango (Magniferaindica)was extracted by soxhlet extraction method using n-hexane as solvent and its chemical and physical properties were evaluated. The chemical parameters investigated include: saponification value (SV), iodine value (IV), peroxide value (PV) and Acid value (%AV). These were found to be85.3∓0.05mgKOH/g, 39.5∓0.10g I2/100gand 4.4mEq/kg and 2.4∓0.01mgKOH/g, respectively. The physical parameters evaluated include: percentage yield (10.21%), relative density (0.87), refractive index (1.4784) and moisture content (12.5%). Soap was then formulated using the oil and the properties of the product were evaluated. From the results, it was found that the products compared favorably to similar products sold in the market in terms of pH, colour, percentage alkali and solubility in water.

Keyword: Oil, Extraction, Chemical, Physical, Parameters, Evaluation


IMPACT OF MERGER AND ACQUISITION ON THE PERFOMANCE OF DEPOSIT MONEY BANKS IN NIGERIA

Osifalujo, Babatunde Bunmi and Olufemi, O Omotilewa

Department of Accountancy

Moshood Abiola Polytechnic, Abeokuta Ogun State

Email: osifalujobabatunde@gmail.com

ABSTRACT

 Low capital base, insolvency, and business distress among other factors have contributed to recent failure of banks in Nigeria. Banks all over the world now adopt mergers and acquisitions as a strategy to improve their performance. This study examined the impact of mergers and acquisition on the performance deposit money bank in Nigeria. The research used capital structure, asset profile, total deposit and profit after tax of the selected bank to measure the performance and effect of merger and acquisition of the bank in both pre and post merger and acquisition period. Data were collected from the published financial statements of the bank namely former Intercontinental Bank Plc and Access Bank (now Access Bank Plc) from 2005 to 2017 and the model was formulated using ordinary least square method. It was revealed that For both the pre-merger and post-merger periods, it was revealed that the access bank performed better. In the post – merger and acquisition period as asset profile and total deposit has no significant effect on the profit after tax of access bank in Nigeria, while capital structure has a significant effect on profit after tax of access bank plc.  While in the pre-merger and acquisition capital structure, asset profile and total deposit have no significant impact on profit after tax of access bank plc. The study concludes that mergers and acquisitions have a significant impact on the performance of deposit money bank in Nigeria. Therefore, the study recommended that banks can merge or acquire each other , as this has proved to become a strategy for rescuing ailing or weak ones and could provides a platform that would enhance financial performance

Keywords: Merger, Acquisition, Financial Performance, Deposit Money Banks.


EXPERIMENTAL STUDY OF THE EFFECT OF DETERGENT CHARACTERISTICS VARIATION ON USERS’BRAND LOYALTY AMONG UNDERGRADUATE STUDENTS OF THE UNIVERSITIES IN MAKURDI METROPOLIS

1Kwahar Nguwasen & 2Onov Phillip

1Department of Business Administration, Federal University of Agriculture, Makurdi

2Department of Economics, Benue State University, Makurdi

E-mail: ngu.kwahar@gmail.com/phixoyoung@gmail.com

ABSTRACT

The study was conducted on the analysis of the effect of detergent characteristics variations on brand users’ loyalty among undergraduate students of the Universities in Makurdi metropolis of Benue State. The aim was to analyze detergent characteristics variations combination that will achieve the best users’ brand loyalty in the study area. The 3x3x3 factorial experimental design was used for the study. This involves three independent variables each varied in three dimensions. The population of the study comprised all users of detergents among undergraduate students of the Universities in Makurdi metropolis of Benue State. These Universities are the Federal University of Agriculture Makurdi and Benue State University Makurdi. The sample of 270 was selected from the target population: 135 students from Federal University of Agriculture Makurdi and 135 students from Benue State University Makurdi. An 18-item self-developed instrument: “Detergent Users Loyalty Inventory” (DULI) was used for data collection. Three-way Analysis of Co-variance (ANCOVA) was used to test the Hypotheses by comparing the effect of the differences in product characteristics variation on brand loyalty. Pre-manipulation scale scores were used for pre-test or as covariate. In particular, the independent measures (between-groups) ANCOVA was used since the study dealt with different subjects under different conditions. The main and interactional effects in ANCOVA result were all significant; the study therefore went further to make use of Fisher’s Least Significant Difference (LSD) to locate the mean differences and sizes. The study found that the overall best brand loyalty level can be achieved only when there is a simultaneous increase in price, size and quality (Mean =178.01). Similarly, other economically optimal brand loyalty levels can be achieved at maintained size, price decrease and quality increase (Mean=177.80) and maintained size, maintained quality and price decrease (Mean =177.30). It was however observed that consumers of detergents in Makurdi metropolis of Benue State responses were in line with the tradition of maximum utility at lowest cost which though good for the rational users, is detrimental to the sustainability of the business.  Detergent Users want both quality and size increase as prerequisite for price increase. This though quite plausible to detergent users, can only be achieved at a loss to the producers. To solve market disequilibrium problems, the study recommended mutual benefits to both the producers and users of detergent. Thus, in view of precarious economic conditions, producers should increase the quality of detergents and the decrease size as prerequisite for price increase(Mean=90.81).This, though with low loyalty level vis-à-vis the best loyalty levels, will be mutually beneficial as it will attract a significant level of loyalty from the detergent users and at the same time, provide a window for producers to achieve high level of profitability.

Keywords: Product Characteristics, Product Characteristics Variation, Brand Loyalty, Detergents Users, Makurdi Metropolis.


PRODUCT DEVELOPMENT AND COMPANY PROFITABILITY FOR SUSTAINABLE DEVELOPMENT OF A NATION IN THE 21ST CENTURY

Saidu Aliyu Baba, Bello Bababtunde Sikiru

and Abdullahi Ibrahim

Department of Marketing

School of Business and Management

Federal Polytechnic, P.M.B 55 Bida

Email:saidualiyubaba@gmail.com

ABSTRACT

It is said and believesthat nothing happens until something is sold, that is true to an extent, and something has to be has to be produced; therefore one talks of selling something. The significance of new product cannot be overemphasized for company’s profitability, because, needs, wants and desire of consumers keep changing overtime, also products pass through some stages in their life, which at diminishing stage, consumer no longer patronize them, leading to profit erode, as such, companies must either produce new products, by abandoning old ones, renovate them to meet the changing needs or enter new market segment (i.e. turnaround), that is when the required profit can be generated and companys growth and national development be achieve, on this note, this paper intends to highlight on the significance or benefits of producing new product for companys growth and national development. From the write up, it was discovered that developing new product by companies enables them to grab investment opportunities, produce the require product to meet needs and desires of the target market within their limited resources and leads to the development of a nation in several ways such as, sources of employment, provision of infrastructural facilities, the writer therefore makes the following recommendations, establishment and research and development department by the company should be a priority, companies should adopt turnaround strategy in the life cycle of a product and effective survey of competitors strategies.


PERFORMANCE OF LAYING JAPANESE QUAILS (Coturnix coturnix japonica) FED DIETS CONTAINING FERMENTED MANGO (mangifera spp) KERNEL COMPOSITE MEAL AS REPLACEMENT FOR MAIZE

Yerima Shettima Kolo1, 1Hamber Terkura Samuel & Hannatu Charles2

1Department of Animal Production, Federal University of Agriculture, Makurdi, Benue State, Nigeria.

2Department of Animal Science, Federal University, Gashua, Yobe State, Nigeria.

ABSTRACT

A study was conducted to investigate the effect of FMKCM on the performance of laying Japanese quails. One hundred and ninety five Japanese quails were randomly allotted to five dietary treatments (I –V) of 39 hens each. Each treatment was replicated thrice with 13 hens per replicate. In each of the five diets, FMKCM was used to replace maize at 0%, 10%, 15%, 20% and 25% for i, ii, ii, iv, and v respectively. Quails in this study were fed over a period 84 days. Feed intake was measured daily and the number of egg laid per replicate per day was recorded. Hen day egg production, hen house egg production, mean daily feed intake show no significant (P>0.05) difference across the treatment. The results obtained on efficiency of feed utilization differed significantly (P<0.05) across treatment groups. Quails placed on 20% and 25% inclusion levels of FMKM were best converter of feed to eggs. It was equally observed that treatment influenced time of lay, with early lay as levels of Fermented mango kernel meal increased in the diets of laying quails.

Keywords: Laying performance, Japanese quails, fermented mango kernel, maize


ASSESSMENT OF THE HAEMATOLOGICAL INDICES OF HbAS AND STEADY STATE HbSS SUBJECTS RESIDENT IN ABUJA, NIGERIA.

1Obiechina MC, 2Sanya JO, 1Okpala S, 3Nwozor CM

1Department of Physiology, University of Nigeria, Enugu campus

2Department of Physiology, College of Medicine and Health Sciences, Afe Babalola University, Ado Ekiti, Ekiti State.

3Department of Physiology, Chukwuemeka Odumegwu Ojukwu University, Uli campus, Anambra State.

ABSTRACT

Sickle cell trait (HbAS) has been regarded as a benign state, whereas there have been reported cases of morbidity associated with HbAS individuals inform of complications and crises. There is an apparent lack of awareness of the likely morbidity of these individuals. This study was conducted to compare the similarities in the hematological Indices of subjects with hemoglobin genotypes AS and Steady State SS. An outpatient population-based study was carried out in Maitama District Hospital, Maitama, Abuja and Kubwa General Hospital, Kubwa, Abuja. The HaemoglobinSS (HbSS), Red Blood Cell count (RBC), Pack Cell Volume (PCV), Mean Corpuscular Volume (MCV), Mean Corpuscular HaemoglobinSS (MCH) and Mean Corpuscular HaemoglobinSS SS Concentration (MCHC) and platelet (PLT) were measured.168 subjects in the age group 10-40 participated in the study, which included 56 HbAS, 46 steady state HbSS and 66 HbAA control. There was no significant differences between the hematological indices of subjects with hemoglobin genotypes AS and Steady State SS in this study. The result of this study will channel the focus of clinicians and researchers to the likelihood of HbAS being a diseased state and further pay more attention to the health status of HbAS patients.

Key words: HbAS, steady state HbSS, Abuja, hematological indices, assessment.


HAEMATOLOGY AND BIOCHEMICAL INDICES OF BROILER FINISHER CHICKEN FED GRADED LEVELS OF ZIZIPHUS MAURITIANA FRUIT PULP

B.J. Mufwa; A.A Maigari; I. Baya,

Department of Animal Production,

College of Agriculture, Jalingo, Taraba State – Nigeria

ABSTRACT

An experiment was conducted to investigate the replacement of maize with ziziphusmauritiana fruit meal in broiler finisher diets. One hundred and twenty Anak 2000 broilers were used for the studym four diets were formulated using ZMFM at 0%, 5%, 10% and 15% in the diets respectively the birds were randomly allotted to dietary treatments in a completely randomized design. Each treatment consult of thirty birds with ten birds per replicate. The experiment lasted for four weeks. Feed and water were given ad-libitum. The Haematological indices indicates high significant (P<0.05) difference at different levels of maize replacement with ZMFM in packed cell volume (PCV), Red blood cell (RBC) Haemaglobin (Hb) while blood cell (WBC), mean corpuscular Haemaglobin (MCH) Haemoglobin concentration (HB)Heterophils and Lymphocytes. The serum biochemical indices revealed high significant (P<0.05) difference in total protein, albumen, glucose, chloride, potassium and sodium. In view of above, up to 15% replacement of maize with ziziphusmauritiana fruit meal has no adverse effect on the blood component of broiler finisher chicken.

Keywords: Broiler finisher, ziziphusmauritiana fruit meal maize and blood components.


INFLUENCE OF EXTENSION COMMUNICATION ON CASSAVA FARMERS’ ADOPTION OF SUSTAINABLE AGRICULTURAL TECHNOLOGIES IN OBUDU LOCAL GOVERNMENT AREA OF CROSS RIVER STATE – NIGERIA

1Adie, U.B. 2Ayi. N.A.  3Ujong O.O.

Department of Agricultural Economics and Extension,

Cross River University of Technology (CRUTECH), Calabar – Obubra.

E-mail address: adieubi67@gmail.com

ABSTRACT

This study analyze the influence of extension communication on cassava farmers’ adoption of sustainable Agricultural Technologies in Obudu Local Government Area of Cross River State – Nigeria.  In recent times, issues of sustainability and natural resources conservation and management have been emphasized globally, and Nigeria has not been an exception.  These issues are of importance in Nigeria’s agricultural sector development.

The specific objectives were to identify the sustainable agricultural technologies and existing extension communication channels, and how it will provide cassava farmers the necessary information and techniques for sustainable agriculture.  The study made use of 100 cassava farmers that were randomly selected from 10 communities in the study area to provide data through the use of structural questionnaire.  Data were analyzed using simple frequency square statistics.  The study revealed that in the study area (Obudu local government area) mixed cropping and organic agriculture, contact with neighbors/family and television has a positive influence on cassava farmers’ adoption of technologies.  The result equally reveals from the analysis that the calculated x2 value was greater than the critical value at the degree of 2 and at 0.5 level of statically significance.

Therefore it means the null hypothesis was rejected, meaning that there was a positive significant relationship between channels of communication and adopted technologies.

It is therefore recommended that farmers’ experiences and indigenous knowledge should be harnessed through participatory extension for a sustainable agricultural development.

Keywords: Adoption, Technologies, Sustainability, Communication.


Recent Comments

    Categories