LYCOPERSICON ESCULENTUM STEM AND EOOT FRACTIONS ATTENUATE ETHIDIUM BROMIDE-INDUCED BIOCHEMICAL ALTERATIONS IN RATS
aSulaiman A. Faoziyat, aAdeotiOluwatomiwa, bSulaiman A.Afolabi, *cOlatundeAhmed,aAbubakar F. Aluko,aOsejiFavor, aOmopupaSuliat, aOdetoyeOmolara, aBamidele B. Eniola, aZakariyah R.Toyin, aNurudeen A. Damilola
aDepartment of Biochemistry, Faculty of Life Sciences,
University of Ilorin, Ilorin
bFederal Medical Center, EbuteMetta, Lagos
cDepartment of Biochemistry
AbubakarTafawaBalewa University, Bauchi740272
Email: olatundebch@gmail.com
ABSTRACT
Ethidium bromide is usually applied as a marker for identifying nucleic acids bands during electrophoretic processes. The information on the ability of natural products to ameliorate the toxic effect this chemical is still scanty. However, this study investigated the ameliorative effect of Lycopersiconesculentum in ethidium bromide induced toxicity in experimental rats.Experimental animals (50) with average body weight of 95±10g were randomly assigned into 10groups each consisting of five each. The normal group was administered with distilled water as the vehicle. The remaining groups of rats were treated with 0.5 ml (2.1 mg/kg body weight) on the skin for two weeks. The standard group was administered with 4.0 mg/kg Contiflo XL while the remaining ethidium bromide treated groups were administered with fractions (aqueous, butanol, ethyl acetate and hexane) of Lycopersiconesculentum stem and root.The results showed that the activities of AST, ALT, GGT, ALP and concentrations of total protein, albumin, bilirubin, creatinine, urea, uric acid, triglyceride, and total cholesterol were altered in the groups treated with ethidium bromide solution compared to the control group. But, Lycopersiconesculentum stem and root fractions (aqueous, butanol, ethyl acetate and hexane) administration ameliorated the alterations in ethidium bromide treated rats.The data from the study indicated that aqueous, butanol, ethyl acetate and hexane fractions of Lycopersiconesculentum stem and rootattenuated the toxic effect of ethidium bromide on the liver, kidney and heart ofrats.
Keywords:Lycopersiconesculentum, toxicity, biochemical indices, fractions