Yabaya
A1* and E. D. Silas2
1Department
of Microbiology, Kaduna State University, Kaduna
2Nationa
Research Institute for Chemical Technology, Basawa, Zaria
amosyabaya@gmail.com
ABSTRACT
Antibacterial
activities of the crude extract of Acacia nilotica seeds using methanol,
chloroform, ethyl acetate and water as solvents were tested on Klebsiella
pneumoniae, Shegella dysenteriae and Staphylococcus aureus. These
strains were clinical isolates and confirmatory biochemical test were
conducted. The phytochemical analyses of the seed extracts indicated the
presence of alkaloids, flavonoids, glycosides and steroids. Disc diffusion
method was used to examine the bacterial susceptibility of the extracts. There
were various zones of inhibition by the different extracts. Methanolic extract
inhibited the growth of Klebsiella
pneumoniae at 90 and 120 mg/ml (2.0 and 4.3mm respectively), Shegella dysenteriae was inhibited at 60
to 120 mg/ml (1.0, 3.5 and 4.1 mm respectively).Staphylococcus aureus was inhibited at all the concentrations. The
trend of extract inhibition was similar in the chloroform and ethyl acetate
while the water extract inhibited the three microorganisms at all the four
levels of concentrations. There is an indication that the plant could be used
to advanced medical practice.
Key
words: Acacia nilotica, Phytochemical,
Antibacterial activity, Seed extracts
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