Table of Contents

Assessment of the Cost of Illness among Patients with Diabetic Foot Ulcer

Statistical Analysis on Reported Cases of HIV/AIDS (A Case Study

 of Special Hospital, Sokoto)

Ibrahim Abubakar Zarumi, Abdullahi Muhammad Sani and Kabiru Malam

Yakubu      1-13                                                                                                                   

Mineral, Vitamin and Sensory Properties of Some Commonly

Consumed Beverages In Abeokuta, Ogun State, Nigeria

Basorun O.P, Agemo C.O, Oluseye A.E   14-30                                                          

Evaluation of Some Selected Plant Extracts against Halitosis Causing Organisms

Fatai Abiola Lateef, Blessing Uloma Eze, Zacchariah Egbunu, Paul Jere and

 Danlandi Yusuf                             31-57                                                                    

Molecular Detection of Hexokinase 1 Gene in the Bloodstream Form

of Trypanosoma Brucei Brucei (Federe Isolate)

Ishaya Y. Longdet and Rotimi J. Ojo 58-71                                                               


MOLECULAR DETECTION OF HEXOKINASE 1 GENE IN THE BLOODSTREAM FORM OF TRYPANOSOMA BRUCEI BRUCEI (FEDERE ISOLATE)

Ishaya Y. Longdet1* and Rotimi J. Ojo1,2

1Department of Biochemistry, University of Jos, Plateau State, Nigeria

2Department of Biochemistry, Bingham University, Karu, Nasarawa

email: islongdet@yahoo.com; longdeti@unijos.edu.ng

ABSTRACT

Hexokinase 1 (EC:2.7.1.1) which belongs to the Hexokinase 2 superfamily is the first key regulatory enzyme in the glycolytic pathway of Trypanosoma brucei brucei. Therefore the enzyme has great significance in metabolism of the parasite particularly for the purpose of generating ATP and nucleic acid precursors. There is increasing interest in the study of enzymes in parasites for various purposes. This research therefore attempted to detect the presence of the hexokinase 1 gene in Trypanosoma brucei brucei (Federe isolate) which is prevalent in Nigeria. The parasites were grown in rats, harvested and purified using diethyl amino ethyl (DEAE) cellulose chromatography. From the genomic DNA isolated the hexokinase 1 gene was amplified through Polymerase Chain Reaction. The amplicon was sequenced and studied using some Bioinformatics tools. The 1401bp gene sequence (Accession number MH 198230) obtained translated into 464 amino acid sequence.  The primary nucleotide sequence of the gene compares well with Trypanosoma brucei TREU 927 and T. brucei gambienseDAL972.The predicted molecular mass of the polypeptide as calculated using ExPASy is 51,918.50Da (~52kDa) giving the hexameric protein a molecular weight of around 311.5kDa which  differs from the mammalian hexokinase The Isoelectric point (pI) was predicted as 9.64indicating that atneutral pH, the enzyme has a netpositive charge. These revealed that the T.b.bruceihexokinase 1 is active in the bloodstream form of the parasite.


EVALUATION OF SOME SELECTED PLANT EXTRACTS AGAINST HALITOSIS CAUSING ORGANISMS

Fatai Abiola Lateef, Blessing Uloma Eze*,  Zacchariah Egbunu, Paul Jere and Danlandi Yusuf

Department of Science Laboratory Technology,

Federal Polytechnic P.M.B.1037, Idah, Kogi State, Nigeria.

e-mail: visituloma@gmail.com

ABSTRACT

Halitosis or bad breath is an unpleasant or offensive odour of the mouth emanating from oral cavity present on exhaled breath. According to medical experts, this very embarrassing condition which can be acute or chronic could be caused by several factors including food, poor dental hygiene, tobacco products, health problems, dry mouth, infections, and medications. Halitosis is detrimental to one’s self-image and confidence causing social, emotional and psychological anxiety. Some food products (barley tea, green tea etc) and plant extracts (Eucalyptus, ginger, aloe vera and coconut) are claimed to be effective in controlling halitosis. To this notice, investigation of some selected plant extracts is conceived as an efficient way to control halitosis. This study was carried out to investigate the mixed extract of Eucalyptus globulus, ginger, aloe vera and coconut oil for the control of halitosis. Organolyptic measurements were used to check for the presence of halitosis. There were 24 volunteers from Science laboratory Technology Federal Polytechnic Idah, aged between 20 to 25 years old. The result of the first examiner before administration of the mouth wash showed that 3(12.5%) absence, moderate and strong malodour, 2(8.30%) had questionable malodour, 6(25.00%) had slight malodour,  and 7(29.16%) had severe malodour whereas the result after the administration of the mouth wash showed that 14(58.33%) had absence of malodour, 4(16.67) had questionable and slight malodour, 0(0.00%) had moderate and strong malodour and 2(8.30%) had strong malodour. The result of the second examiner before the administration of the mouth wash showed that 3(12.50%) had absence, questionable, moderate and strong malodour, 5(20.83%) had slight malodour and 7(29.16%) had severe malodour  whereas the result after the administration of the mouth wash showed that 14(58.33%) had absence of malodour, 2(8.30%) had questionable malodour, 5(20.83%) had slight malodour, 0(0.00%) had moderate, strong and severe malodour. The antimicrobial activity of the mixed extract against Escherichia coli, Staphylococcus aureus, Pseudomonas aeurginosa and Klebsiella pneumonia was determined and their zones of inhibitions were 16, 17, 14 and 13 mm respectively at 80ul. The mixed extract was active against the microorganism which are causative agent of halitosis in vitro. There was great reduction in the number of volunteers who had halitosis when the mixed extract was administered. So the mixed extract was effective against the microorganisms which cause halitosis in vivo. It was also able to give fresh breath and reduce oral malodour  


MINERAL, VITAMIN AND SENSORY PROPERTIES OF SOME COMMONLY CONSUMED BEVERAGES IN ABEOKUTA, OGUN STATE, NIGERIA

1Basorun O.P, 2Agemo C.O, 3Oluseye A.E

1Pharmaceutical Technology Department, Moshood Abiola Polytechnic Ojere, Abeokuta,

2Food Technology Department, Moshood Abiola Polytechnic Ojere, Abeokuta

3Nutrition and Dietetics Department, Moshood Abiola Polytechnic Ojere, Abeokuta

Email:basorunomobola2005@gmail.com

ABSTRACT

Beverages have played an important role in the diet of Nigerians. This study is designed to determine the mineral, vitamin of sensory value of selected beverage in Abeokuta. Mineral, Vitamin and Sensory evaluation of the beverages were determined using standard method. The result of the mineral composition shows that iron, potassium, calcium, magnesium, selenium and sodium values ranged from 6.15 to 8.75, 146.15 to 123.1, 1.58 to 1.76, 1.44 to 1.13, 4.93 to 4.82 and 0.63 to 0.44 in mg/100ml respectively. The results of selected vitamins were 8.28, 0.179, 0.434. 1.97 and 12.9 respectively. The results of the sensory evaluation were observed in all the teas with difference attributes of taste, odour, colour and overall acceptability. Beverage could give mineral and vitamin to the consumer if taken in right proportion.


STATISTICAL ANALYSIS ON REPORTED CASES OF HIV/AIDS (A CASE STUDY OF SPECIAL HOSPITAL, SOKOTO)

Ibrahim Abubakar Zarumi, Abdullahi Muhammad Sani and Kabiru Malam Yakubu

Department of Mathematics & Statistics

Umaru Ali Shinkafi Polytechnic, Sokoto,

Emails:ibrahimabubakarzarumi@gmail.com, abdullahsani7@gmail.com, kabeerygat@yahoo.com

ABSTRACT

The paper examined the reported cases of HIV/AIDS infected patients within the periods of 2007 to 2016 at Specialist Hospital Sokoto (SHS), Sokoto State, Nigeria. The Statistical tools used for the project were Pearson’s Product Moment Correlation Coefficient (PMCC), Student’s t-test for difference of two independent sample means and Chi-square test for independence. From the analysis it was observed that there is no significant relationship between male and female patients infected with HIV/AIDS. Also, there is no significant difference in the average number of male and female patients infected with HIV/AIDS attending SHS and the  2-test shows that HIV/AIDS infection diseases depend on sex (i.e. male and female).


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