Akinboboye, O.A1., Adenekan, M.O1., Olubode, T2., Ikuejawa, I. I3 & Onasanya, R.O4.
1Department of Microbiology, Southwestern University, Okun-Owa,
Ogun State.
2Department of Home and Rural Economics, Fed. Coll. of
Agriculture, Moor Plantation, Ibadan
3Department of Agric. Ext. and Management, Fed. Coll. of
Agriculture, Moor Plantation, Ibadan
4Department of Agriculture, Fed. College of Agriculture, Moor
Plantation, P. M. B. 5029, Ibadan.
E-mail: omolikiarinola@gmail.com; mopadenekan@yahoo.com
ABSTRACT
Enteropathogenic Escherichia coli is one of
the most important pathogens causing severe and persistent watery diarrhoea,
abdominal pain, fever, vomiting and often lead to death in humans. Diarrhoeic faecal samples were collected from 210 children
(aged < 5 years) in three
different hospitals which are Federal Medical centre Idi-Aba Abeokuta, Otunba Tunwase Padectrics Centre, Ijebu Ode and Olabisi Onabanjo
Teaching hospital Sagamu after questionnaires were administered. The stool samples were cultured for bacteria on Eosin
methylene blue agar, MacConkey agar, and Salmonella-Shigella agar. Escherichia coli were identified
using biochemical characterization. Enteropathogenic Escherichia coli were serotyped by slide agglutination test using specific Enteropathogenic Escherichia coli
monovalent antisera (EPEC O111, O126, O86, O55, O128 and O26). Antibiotic
sensitivity was carried out using disc diffusion method. Plasmids from
multi-drug resistant Enteropathogenic Escherichia
coli were extracted by Alkali-lysis method and electrophoresed.
Genomic DNA were extracted from Enteropathogenic
Escherichia coli and subjected to multiplex Polymerase Chain Reaction for detection of effacing
and attaching (eae),
bundle-forming pili (bfp) and
enteropathogenic Escherichia coli
adherence factor (eaef) genes. The strains that exhibited multiple drug resistant were 21(18.8%) with plasmids detected in
12(57.1%) of the 21 multi-drug resistance Escherichia coli. Twenty-one of the Enteropathogenic Escherichia coli strains possessed effacing and attaching genes, 6 possessed
bfp while 18 possessed eaef. Typical Enteropathogenic Escherichia coli
strains were more prevalent (tEPEC)
with (71.4%) than atypical (aEPEC) (28.6%). The molecular
characterization of the Enteropathogenic Escherichia
coli among children in this study, revealed typical Enteropathogenic Escherichia
coli (tEPEC) as the dominant strain in Ogun state.
Keyword: Multidrug,
Enteropathogenic E. coli, Prevalence,
Gastroenteritis, Typical Enteropathogenic E.
coli,
Recent Comments